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LU

Emma Hammarlund

Research team manager

LU

Hypoxia inducible factor-2α importance for migration, proliferation, and self-renewal of trunk neural crest cells

Author

  • Camilla U. Niklasson
  • Elina Fredlund
  • Emanuela Monni
  • Jessica M. Lindvall
  • Zaal Kokaia
  • Emma U. Hammarlund
  • Marianne E. Bronner
  • Sofie Mohlin

Summary, in English

Background: The neural crest is a transient embryonic stem cell population. Hypoxia inducible factor (HIF)-2α is associated with neural crest stem cell appearance and aggressiveness in tumors. However, little is known about its role in normal neural crest development. Results: Here, we show that HIF-2α is expressed in trunk neural crest cells of human, murine, and avian embryos. Knockdown as well as overexpression of HIF-2α in vivo causes developmental delays, induces proliferation, and self-renewal capacity of neural crest cells while decreasing the proportion of neural crest cells that migrate ventrally to sympathoadrenal sites. Reflecting the in vivo phenotype, transcriptome changes after loss of HIF-2α reveal enrichment of genes associated with cancer, invasion, epithelial-to-mesenchymal transition, and growth arrest. Conclusions: Taken together, these results suggest that expression levels of HIF-2α must be strictly controlled during normal trunk neural crest development and that dysregulated levels affects several important features connected to stemness, migration, and development.

Department/s

  • LUCC: Lund University Cancer Centre
  • Division of Translational Cancer Research
  • Stem Cells & Restorative Neurology
  • Neural stem cell biology and therapy
  • Neurology, Lund
  • StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
  • Stem Cell Center
  • EpiHealth: Epidemiology for Health
  • Childhood Cancer Research Unit
  • Paediatrics (Lund)

Publishing year

2021

Language

English

Pages

191-236

Publication/Series

Developmental Dynamics

Volume

250

Issue

2

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Cell and Molecular Biology

Keywords

  • embryogenesis
  • HIF-2α
  • migration
  • neural crest
  • stem cells
  • trunk neural crest

Status

Published

Research group

  • Stem Cells & Restorative Neurology
  • Neural stem cell biology and therapy
  • Childhood Cancer Research Unit

ISBN/ISSN/Other

  • ISSN: 1058-8388