Emma Hammarlund
Research team manager
Hypoxia inducible factor-2α importance for migration, proliferation, and self-renewal of trunk neural crest cells
Author
Summary, in English
Background: The neural crest is a transient embryonic stem cell population. Hypoxia inducible factor (HIF)-2α is associated with neural crest stem cell appearance and aggressiveness in tumors. However, little is known about its role in normal neural crest development. Results: Here, we show that HIF-2α is expressed in trunk neural crest cells of human, murine, and avian embryos. Knockdown as well as overexpression of HIF-2α in vivo causes developmental delays, induces proliferation, and self-renewal capacity of neural crest cells while decreasing the proportion of neural crest cells that migrate ventrally to sympathoadrenal sites. Reflecting the in vivo phenotype, transcriptome changes after loss of HIF-2α reveal enrichment of genes associated with cancer, invasion, epithelial-to-mesenchymal transition, and growth arrest. Conclusions: Taken together, these results suggest that expression levels of HIF-2α must be strictly controlled during normal trunk neural crest development and that dysregulated levels affects several important features connected to stemness, migration, and development.
Department/s
- LUCC: Lund University Cancer Centre
- Division of Translational Cancer Research
- Stem Cells & Restorative Neurology
- Neural stem cell biology and therapy
- Neurology, Lund
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Stem Cell Center
- EpiHealth: Epidemiology for Health
- Childhood Cancer Research Unit
- Paediatrics (Lund)
Publishing year
2021
Language
English
Pages
191-236
Publication/Series
Developmental Dynamics
Volume
250
Issue
2
Document type
Journal article
Publisher
John Wiley & Sons Inc.
Topic
- Cell and Molecular Biology
Keywords
- embryogenesis
- HIF-2α
- migration
- neural crest
- stem cells
- trunk neural crest
Status
Published
Research group
- Stem Cells & Restorative Neurology
- Neural stem cell biology and therapy
- Childhood Cancer Research Unit
ISBN/ISSN/Other
- ISSN: 1058-8388